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Image Processing with ImageJ - Second Edition

Image Processing with ImageJ - Second Edition

By : Jurjen Broeke, Pascau
3.4 (5)
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Image Processing with ImageJ - Second Edition

Image Processing with ImageJ - Second Edition

3.4 (5)
By: Jurjen Broeke, Pascau

Overview of this book

Advances in image processing have been vital for the scientific and technological communities, making it possible to analyze images in greater detail than ever before. But as images become larger and more complex, advanced processing techniques are required. ImageJ is built for the modern challenges of image processing – it’s one of the key tools in its development, letting you automate basic tasks so you can focus on sophisticated, in depth analysis. This book demonstrates how to put ImageJ into practice. It outlines its key features and demonstrates how to create your own image processing applications using macros and ImageJ plugins. Once you’ve got to grips with the basics of ImageJ, you’ll then discover how to build a number of different image processing solutions. From simple tasks to advanced and automated image processing, you’ll gain confidence with this innovative and powerful tool – however and whatever you are using it for.
Table of Contents (12 chapters)
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2
2. Basic Image Processing with ImageJ
11
Index

Correcting images

In order to analyze images, we sometimes need to correct the problems that were present during acquisition. Problems such as noise, uneven illumination, and background fluorescence can cause many issues during image analysis. I will provide a little technical background on the sources of these problems and then follow this up with how they can be corrected in ImageJ.

Technical background

Of the many sources of noise that exist in imaging, a few can be corrected with correct acquisition settings. Others are inherent in the electronics and physical properties of the camera, and cannot be easily fixed. I will first deal with the source of noise that can be remedied with optimizing acquisition: Shot or Poisson noise. Next, we will look at Electronic or Dark noise.

Correcting Shot noise

Shot noise is caused by the physical properties of light; light can be seen as packages of light or photons. The number of photons that are collected by each photo-detector site on the camera determines...

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